sushabhan | IIT Palakkad

Biosketch
Positions:
- Associate Professor
  Department of Chemistry, Indian Institute of Technology Palakkad, India
  Duration: June 2023 - present
- Assistant Professor
  Department of Chemistry, Indian Institute of Technology Palakkad, India
  Duration: July 2019 - May 2023
- Assistant Professor
  Discipline of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, India
  Duration: May 2017 - June 2019
- Postdoctoral Associate
  Department of Chemistry and Chemical Biology, Cornell University, New York, USA
  Duration: Sept 2012 - April 2017
Educations:
- Case Western Reserve University, Cleveland, Ohio, USA
  Ph.D. in Chemistry (2012)
- Indian Institute of Technology Delhi, New Delhi, India
  M.Sc. in Chemistry (2007)
- University of Calcutta, West Bengal, India
  B.Sc. in Chemistry (Hons.) (2005)
Research

Structural and functional investigation of natural products for biomedical applications: Natural products have long served as a bedrock of drug discovery. Our group is dedicated to exploring a variety of these molecules and transforming them into potential drug candidates. Our primary focus is on unraveling the molecular mechanisms underlying the diverse biological activities of these compounds, utilizing chemoproteomics as a powerful investigative tool. In addition, we leverage our chemical synthesis platform to structurally modify these molecules, aiming to develop potent inhibitors for various proteins/enzymes involved in the prognosis of a wide range of diseases. Beyond drug discovery, we are also interested in exploring the biomaterial potential of these natural products.

Development of natural product-inspired as well as synthetic antimicrobial agents against antibiotic-resistant pathogens: Our research group focuses on the development of novel antibacterial agents to combat multidrug-resistant (MDR) bacteria. We harness the therapeutic potential of natural products, particularly plant-derived polyphenols, known for their excellent safety profiles and broad-spectrum bioactivity. In addition to natural compounds, our work extends to the design and synthesis of small molecules and the development of innovative metal-based nanoformulations. Through a multidisciplinary approach, we aim to discover and optimize next-generation antimicrobials to address the global challenge of antibiotic resistance.

Targeting diabetes through polyphenol-based small molecule inhibitors with multi-target activity: Natural products offer immense structural diversity and serve as a rich source of biologically active compounds. Among them, polyphenols stand out for the wide range of multi-therapeutic effects with low cytotoxicity. However, it has several bottlenecks and is ineffective when used directly as a medication. Diabetes, being a multifactorial disease, a single drug targeting a single biological factor is outdated, and it requires a multi-targeting antidiabetic agent. Our lab hereby focused on exploring the multi-therapeutic potential of polyphenols to overcome the bottlenecks while enhancing their efficacy by structural modifications on them.

Chemo-proteomics approaches for drug-target interactions using Click chemistry: Chemo-proteomics combines chemistry and proteomics to study how small molecules, like drugs, interact with proteins in living systems. Our group develops small molecule inhibitors and chemical probes to selectively target and label specific proteins in complex biological samples. We investigate small molecule–protein and protein–protein interactions in cells to understand their roles in cellular pathways and diseases. Using techniques such as mass spectrometry, affinity chromatography, gel electrophoresis, and immunoblotting, we study these interactions at the cellular level. We also design bio-orthogonal probes for natural compounds to uncover their mode of action using Cu(I)-catalyzed alkyne-azide Click chemistry

Understanding protein post-translational modifications (PTMs) and their enzymatic regulators for therapeutic applications: Another area where we are actively working is understanding protein post-translational modifications (PTMs) and their enzymatic regulators, with the goal of uncovering insights that can drive the development of new therapeutic strategies. PTMs are closely linked to a range of human diseases, including cardiovascular disorders, diabetes, cancer, and neurodegenerative conditions. Gaining detailed insight into the timing and regulation of these modifications is essential to understand the fundamental biological processes and also for guiding the development of novel therapeutics for serious and life-threatening diseases.
Teaching
At Indian Institute of Technology Palakkad (IIT Palakkad)
- CY 5017: Chemistry of Life (M.Sc. Course; Aug 2020)
- CY 5013: Synthetic Approaches in Chemistry (M.Sc. Course; Aug 2020)
- BT 2010: Life Sciences (B.Tech. Course; Aug 2020)
- CY 1140: Chemistry Lab (B.Tech. Course; Jan 2020)
- CY 5104: Synthesis, Energetics and Dynamics III (M.Sc. Course; Jan 2020)
- CY 1010: Thermodynamics, Kinetics, and Organic Reactions (B.Tech. Course; July 2019)
- BT 2010: Life Sciences (B.Tech. Course; July 2019)
At Indian Institute of Technology Indore (IIT Indore)
- BSE 608: Advanced Drug Delivery Systems (Ph.D. Course; Autumn 2017, 2018)
- BSE 651: Biochemistry Lab (M.Sc. Course; Autumn 2017)
- BSE 102: Biosciences (B.Tech. Course; Spring 2018, 2019)
- BSE 615: Principles of Chemical Biology (Ph.D. Course; Autumn 2018)
Research Group

Ph.D. Students

Mr. Sharif Aslam CP, UGC-JRF

M.Sc. (2021-2023) in Chemistry from the NIT Calicut, Kerala

B.Sc. (2018-2021) in Chemistry from MES Mampad College, Malappuram, Kerala

Joined: Aug 2024

Email: 202414010@smail.iitpkd.ac.in

Ms. Jissy Anna George, HTRA-JRF

M.Sc. (2021-2023) in Biochemistry from University of Hyderabad, Hyderabad

B.Sc. (2018-2021) in Biochemistry from Kerala University, Kerala

Joined: Jan 2024

Email: 172404001@smail.iitpkd.ac.in

Ms. Karthika K, PMRF

M.Sc. (2018-2020) in Chemistry from Govt. Victoria College Palakkad, Kerala

B.Sc. (2015-2018) in Chemistry from Govt. College Chittur, Kerala

Joined: Aug 2022

Email: 202214002@smail.iitpkd.ac.in

Mr. Vishnu NV, HTRA-JRF

M.Sc. (2019-2021) in Chemistry from the University of Calicut, Kerala

B.Sc. (2016-2019) in Chemistry from Sri Vyasa NSS College, Vyasagiri, Kerala

Joined: Aug 2022

Email: 202214009@smail.iitpkd.ac.in

Mr. Shabin NC, CSIR-SRF

M.Sc. (2018-2020) in Chemistry from the University of Calicut, Kerala

B.Sc. (2015-2018) in Chemistry from Govt. Arts & Science College Meenchantha, Kerala

Joined: Aug 2021

Email: 202114002@smail.iitpkd.ac.in

Ms. Revathy S, CSIR-SRF

M.Sc. (2015-2017) in Chemistry from Govt. Victoria College Palakkad, Kerala

B.Sc. (2012-2015) in Chemistry from Govt. Victoria College Palakkad, Kerala

Joined: Jan 2020 (Thesis submitted)

Email: 202004003@smail.iitpkd.ac.in

Ms. Suchi Chaturvedi, DST-Inspire Fellow-SRF (Continuing at IIT Indore)

M.Sc. (2015-2017) in Biochemistry from Devi Ahilya Vishwavidyalaya, Madhya Pradesh

B.Sc. (2012-2015) in Biochemistry from Rani Durgavati Vishwavidyalaya, Madhya Pradesh

Joined: July 2018 (Thesis submitted)

Email: phd1801171002@iiti.ac.in

Alumni

Dr. Satyam Singh (PhD student: 2018-2023; Current Affiliation: Postdoc at Roswell Park Comprehensive Cancer Center, NY, USA)

Dr. Anupama Binoy (Postdoc: 2020-2023; Current Affiliation: Postdoc at Ohio University, OH, USA)

Dr. Debashis Majee (Visiting scientist: 2019-2020; Current Affiliation: Postdoc at Yale-NUS College, Singapore)

Mr. Manan Kothari (Project intern, 2022-2024. Current Affiliation: Ph.D. student, University of Kansas, USA)

Mr. Harsh Rana (M.Sc. project, 2024. Current Affiliation: Not available)

Ms. Illakia Selvan (M.Sc. project, 2024. Current Affiliation: Not available)

Ms. Haritha M. (Project assistant, 2022. Current Affiliation: Ph.D. student, NIT Calicut)

Ms. Nidhi Bharti (Summer Intern, 2022. Current Affiliation: Integrated M.Sc. student, NIT Patna)

Ms. Monit Yadav (Summer Intern, 2022. Current Affiliation: M.Sc. student, NIT Nagpur)

Mr. Ramraj Kameriya (M.Sc. project, 2022. Current Affiliation: Not available) Best M.Sc. Project Award

Mr. Prince Kumar (M.Sc. project, 2022. Current Affiliation: Not available)

Ms. Samridhi Sinha (M.Sc. project, 2022. Current Affiliation: Not available)

Mr. Nikhil Kumar (M.Sc. project, 2021. Current Affiliation: Not available)

Ms. Preeti Kang (M.Sc. project, 2021. Current Affiliation: Ph.D. student at IIT Jodhpur) Best M.Sc. Project Award

Mr. Anurag Pandey (M.Sc. project, 2020. Current Affiliation: Syngene)

Ms. Tripti Kundu (M.Sc. project, 2020. Current Affiliation: Ph.D. student at IIT Dhanbad)

Ms. Rajani Kudumula (M.Sc. project, 2020. Current Affiliation: Not available)

Ms. Irene Shajan (Project assistant, 2019. Current Affiliation: Ph.D. student at Wageningen University, Netherlands)

Ms. Chandana Yesudas (M.Sc. project, 2020. Current Affiliation: Ph.D. student at Tamil Nadu Government University)

Ms. Sai Kumari Vechalapu (M.Sc. project, 2018. Current Affiliation: Ph.D. student at IIT Kanpur)

Mr. Indranil De (JRF, 2017. Current Affiliation: Ph.D. student at INST Mohali)
Additional Information
Title

Selected Journal Publications

Description

Liya Thurakkal^#, Satyam Singh^#, Rajarshi Roy, Parimal Kar, Sushabhan Sadhukhan*, Mintu Porel*. An in-silico study on selected organosulfur compounds as potential drugs for SARS-CoV-2 infection via binding multiple drug targets. Chem. Phys. Lett. 2020, Just accepted. (^#Equal contribution) DOI: 10.1016/j.cplett.2020.138193.

Satyam Singh, Md Fulbabu Sk, Avinash Sonawane, Parimal Kar, Sushabhan Sadhukhan*. Plant-derived natural polyphenols as potential antiviral drugs against SARS-CoV-2 via RNA‐dependent RNA polymerase (RdRp) inhibition: An in-silico analysis. J. Biomol. Struct. Dyn. 2020, Just accepted. DOI: 10.1080/07391102.2020.1796810.

Indranil De, Sushabhan Sadhukhan*. Emerging Roles of DHHC-mediated Protein S-palmitoylation in Physiological and Pathophysiological Context. Euro. J. Cell Biol. 2018, 97, 319-338. Featured on the journal cover page.

Sushabhan Sadhukhan, Xiaojing Liu, Dongryeol Ryu, Ornella Nelson, John Stupinski, Zhi Li, Wei Chen, Sheng Zhang, Robert Weiss, Jason Locasale, Johan Auwerx, and Hening Lin. Metabolomics-assisted Proteomics Identifies Succinylation and SIRT5 as Important Regulators of Cardiac Function. Proc. Natl. Acad. Sci. U S A. 2016, 113, 4320-4325. (Highlighted on many science news outlets including Science Daily, EurekAlert!, Medical News Today, Scicasts)

Sushabhan Sadhukhan, Guo-Fang Zhang, and Gregory P. Tochtrop. Modular Isotopomer Synthesis of γ-Hydroxybutyric Acid for a Quantitative Analysis of Metabolic Fates. ACS Chem. Biol. 2014, 9, 1706-1711.

Sushabhan Sadhukhan, Yong Han, Zhicheng Jin, Gregory P. Tochtrop, and Guo-Fang Zhang. Glutathionylated 4-Hydroxyalkenal Enantiomers in Rat Organs and Its Contributions Towards the Disposal of 4-Hydroxynonenal in Rat Liver. Free Rad. Biol. Med. 2014, 70, 78-85.

Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Using Selective Isotopic Labeling to Quantitate Parallel Metabolic Pathways. J. Am. Chem. Soc. 2010, 132, 6309-631.

Title

Research Funding
Title

Research & Academic Programs Organized
Description
Conducted a short-term course on “Advanced Biological and Chemical Understanding of Disease Diagnostics and Therapeutics” under Continuing Education Program (CEP) (May 17-19, 2018)

Organized a two days international symposium entitled as “Emerging Areas in Biosciences and Biomedical Technologies (eBBT) 2018” at IIT Indore during January 5-6, 2018.

Organized a one-day international symposium entitled as “Water: Resources, Challenges & Sustainability (WRCS)” at IIT Indore on March 10, 2018.
Title

Honors & Awards
Description
‘Early Career Research Award’ from the Science and Engineering Research Board (SERB), 2018

Postdoc Leadership Certificate, Cornell University, Ithaca, NY, USA, 2016.

Outstanding oral presentation at the Meeting in Miniature from American Chemical Society, Cleveland section, Cleveland, OH, USA, 2011.

Conference Travel Award from Gordon Research Conference based on excellent poster presentation, Boston, MA, USA, 2011.

Second prize in Research Showcase, Case Western Reserve University, Cleveland, OH, USA, 2010.

Conference Travel Award from the Division of Biological Chemistry of American Chemical Society, San Francisco, CA, USA, 2010.

Endowment Sponsored Mentorship Award, Case Western Reserve University, Cleveland, OH, USA, 2010.

Merit-Cum-Means (MCM) Scholarship, Indian Institute of Technology Delhi, India, 2005-07.

S. J. Jindal Trust Scholarship, New Delhi, India, 2004.
Title

Professional Services

Description

Member of Board of Research (BoR) of IIT Palakkad (2019-2020)

Institute colloquium organizer at IIT Palakkad (2019-2020)

Biology coordinator for DST-sponsored Vigyan Jyoti Program (May 2018) at IIT Indore

Member Secretary of IIT Indore-Institutional Committee for Stem Cell Research (2017-2019)

Reviewer for the following peer-reviewed journals: (i) Scientific Reports, (ii) Proteomics, (iii) ACS Applied Materials & Interfaces, (iv) PLoS One, (v) Cell Communication and Signaling.

Served as a reviewer for the undergraduate summer funding applications from Support of Undergraduate Research & Creative Endeavors (SOURCE), Case Western Reserve University, Cleveland, OH, USA, 2011.

Served as a judge for the undergraduate poster presentation in SOURCE Symposium & Poster Session, Case Western Reserve University, Cleveland, OH, USA, 2010.

Member of American Chemical Society (ACS), 2010-current

Member of Society of Biological Chemists, India (SBCI), Life member

Title

Selected Oral Presentations

Description

Understanding the Mechanism of Action of (–)-Epigallocatechin-3-gallate (EGCG), A Major Green Tea Polyphenol. Advances at the Interface of Biology & Chemistry, BARC, Mumbai, India, Nov 1-3, 2019.

Protein Post-Translational Modifications (PTMs): Small Changes, Big Impact. Mitochondria & Metabolism Network Meeting, IISER Pune, India, November 10-11, 2017.

Metabolomics-assisted Proteomics Identifies Protein Lysine Succinylation and SIRT5 as Important Regulators of Cardiac Metabolism and Function. 252^nd American Chemical Society National Meeting, Philadelphia, PA, USA, August 21, 2016.

Metabolomics-assisted Proteomics Identifies Succinylation and SIRT5 as Important Regulators of Cardiac Function. Cornell University, Ithaca, NY, USA, November 13, 2015.

Protein modifications: When, how, where and so what? Young Investigator Meeting, Massachusetts Institute of Technology, Boston, MA, USA, October 11, 2015.

A Modular Isotopomer Synthesis of gamma-Hydroxybutyrate (GHB) Reveals Catabolism via Alpha Oxidation. ACS Meeting in Miniature, Ursuline College, Cleveland, OH, USA, March 16, 2011.

A Modular Isotopomer Synthesis of gamma-Hydroxybutyrate (GHB) Reveals Catabolism via Alpha Oxidation. Departmental Retreat, Case Western Reserve University, Cleveland, OH, USA, October 30, 2010.

Metabolism of 4-Hydroxy Acids: Using a Selective Isotope Labeling Strategy. Departmental Retreat, Case Western Reserve University, Cleveland, OH, USA, October 3, 2009.

Title

Selected Conference (poster) Presentations

Description

Sai K Vechalapu, and Sushabhan Sadhukhan*. Understanding the Mechanism of Action of (–)-Epigallocatechin-3-gallate (EGCG), A Major Green Tea Polyphenol. 1^st In-house Symposium on Advances in Biosciences and Bioengineering, Indian Institute of Technology Indore, India, February 23, 2019.

Satyam Singh, Suchi Chaturvedi, and Sushabhan Sadhukhan*. Chemical Biology of DHHC-mediated Protein S-palmitoylation and Dissecting Its Roles in Breast Cancer. Industry-Academia Conclave 2018 (IAC-2018), Indian Institute of Technology Indore, India, November 20, 2018.

Sushabhan Sadhukhan, Xiaojing Liu, Ornella Nelson, John Stupinski, Sheng Zhang, Robert Weiss, Jason Locasale, and Hening Lin. Metabolomics-assisted Proteomics Reveals the Function of Lysine Succinylation and SIRT5 in Regulation of β-Oxidation of Long-chain Fatty Acids. 64^th American Society for Mass Spectrometry Conference, San Antonio, Texas, USA, June 5-9, 2016.

Sushabhan Sadhukhan, Xiaojing Liu, Dongryeol Ryu, Ornella D. Nelson, Hening Lin. Metabolomics-assisted Proteomics Identifies Succinylation and SIRT5 as Important Regulators of Heart Function. Chemistry Biology Interface Symposium, Cornell University, Ithaca, New York, USA, March 7, 2015.

Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Metabolomics of Lipid Peroxidation Products Using a Selective Isotope Labeling Strategy. Gordon Research Conferences (Molecular and Cellular Biology of Lipids), Waterville Valley, New Hampshire, USA, July 17-22, 2011.

Sushabhan Sadhukhan, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Metabolism of Lipid Peroxidation Products Using a Selective Isotope Labeling Strategy. 42^nd National Organic Symposium, Princeton University, New Jersey, USA, June 5-9, 2011.

Sushabhan Sadhukhan, Guo-Fang Zhang, Tonibelle Gatbonton-Schwager, Henri Brunengraber, and Gregory P Tochtrop. The Metabolism and Signaling of the Lipid Peroxidation Product 4-HNE. American Association for Cancer Research and American Chemical Society Joint Meeting, San Diego, California, USA, January 30-February 2, 2011.

Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Metabolism and Signaling of Lipid Peroxidation Products. Gordon Research Conferences (Bioorganic Chemistry), Andover, New Hampshire, USA, June 13-18, 2010.

Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Metabolism of 4-Hydroxyacids and 4-Hydroxynonenal Using a Selective Isotope Labeling Strategy. Research Showcase, Case Western Reserve University, Cleveland, Ohio, USA, April 15, 2010.

Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop. Metabolism of 4-Hydroxyacids and 4-Hydroxynonenal Using a Selective Isotope Labeling Strategy. 239^th American Chemical Society National Meeting, San Francisco, California, USA, March 21-25, 2010
Publications

Unconventional fluorescence behaviour of ε-polylysine: Selective and sensitive detection of Cu2+ in real water samples, test paper strips, living cells, and anticounterfeiting applications
Sahadevan, R.; Kannan, K.; Jose, A.; Porel, M.*; Sadhukhan, S.*
Sens. Actuators B Chem in press 135589 (2024)

Novel molecular hybrids of EGCG and quinoxaline: Potent multi-targeting antidiabetic agents that inhibit α-glucosidase, α-amylase, and oxidative stress
Kothari, M.; Kannan, K.; Sahadevan, R.; Sadhukhan, S.*
Int. J. Biol. Macromol. 263 130175 (2024)

Identification of selective plant-derived natural carotenoid and flavonoids as the potential inhibitors of DHHC-mediated protein S-palmitoylation: an in-silico study
Chaturvedi, S.; Pandya, N.; Sadhukhan, S.; Sonawane, A.*
J Biomol. Struct. Dyn. in press (2024)

Protein S-palmitoylation is markedly inhibited by 4"-alkyl ether lipophilic derivatives of EGCG, the major green tea polyphenol: In vitro and in silico studies
Binoy, A.; Kothari, M. ; Sahadevan, R. ; Poddar, S.; Kar, P.; Sadhukhan, S.*
Biochim. Biophys. Acta Biomembr. 1866 184264 (2024)

Selective luminescent detection of 5-nitroimidazole antibiotics through self-aggregates of a single non-aromatic amino acid, L-lysine
Jose, A.; Vijayan, V. N.; Sahadevan, R.; Porel, M.*; Sadhukhan, S.*
Microchem. J. 197 109802 (2024)

Pyrene aroylhydrazone-based Pd(II) complexes for DNA/protein binding, cellular imaging and in vitro anticancer activity via ROS production
Vignesh, A.; Binoy, A.; Thurakkal, L.; Bhuvanesh, N.S.P.; Sadhukhan, S.*; Porel, M.*
J. Mol. Struct. 1295 136693 (2024)

4"-Alkyl EGCG derivatives induce cytoprotective autophagy response by inhibiting EGFR in glioblastoma cells
Singh, S.; Ghosh, P.; Roy, R.; Behera, A.; Sahadevan, R.; Kar, P.; Sadhukhan, S.*; Sonawane, A.*
ACS Omega 9 2286 (2024)

Mitochondria-targeting EGCG derivatives protect H9c2 cardiomyocytes from H2O2-induced apoptosis: Design, synthesis and biological evaluation
Sahadevan, R. ; Binoy, A. ; Shajan, I.; Sadhukhan, S.*
RSC Adv. 13 29477 (2023)

20 years since the approval of first EGFR-TKIs, Gefitinib: Insight and foresight
Singh, S.; Sadhukhan, S.*; Sonawane, A.*
Biochim. Biophys. Acta: Rev. Cancer 1878 188967 (2023)

Non-aromatic single amino acid, lysine: A simple fluorescence sensor for selective detection of Cu (II) and Co(II) with colorimetric distinction by the virtue of its self-aggregation behaviour
Sahadevan, R.; Jose, A.; Porel, M.*; Sadhukhan, S.*
J. Photochem. Photobiol. A Chem. 445 115086 (2023)

Role of EGFR and FASN in breast cancer progression
Chaturvedi, S.; Biswas, M.; Sadhukhan, S.*; Sonawane, A.*
J. Cell. Commun. Signal. 17 1249 (2023)

Design, synthesis, and applications of pyrene-quinoline based cell-permeable AIEgen for cellular imaging and antibacterial activity
Jose, A.; Chathangad, S. N.; Sahadevan, R.; Binoy, A.; Vignesh, A.; Sadhukhan, S.*; Porel, M.*
J. Photochem. Photobiol. A Chem. 444 114950 (2023)

In situ global proteomics profiling of EGCG targets using a cell-permeable and Click-able bioorthogonal probe
Sahadevan, R., Binoy, A., Vechalapu, S.K., Nanjan, P., Sadhukhan, S.*
Int. J. Biol. Macromol. 237 123991 (2023)

Dansyl appended sequence-defined oligomers for selective ultrasensitive detection of Hg2+ in water, paper strips, living cells and its efficient removal
Jose, A., Sahadevan, R., Vijay, M, Sadhukhan, S.*, Porel, M.*
Sens. Actuators B Chem 380 133335 (2023)

Chemico-biological aspects of (−)-epigallocatechin-3-gallate (EGCG) to improve its stability, bioavailability and membrane permeability: Current status and future prospects
Revathy Sahadevan, Satyam Singh, Anupama Binoy, Sushabhan Sadhukhan*
Crit. Rev. Food. Nutr. Sci. 63 10382 (2023)

A novel 4"-alkyl ether derivative of green tea polyphenol EGCG as potent and selective EGFR inhibitor: In-vitro and in-silico studies
Satyam Singh, Revathy Sahadevan, Rajarshi Roy, Mainak Biswas, Priya Ghosh, Parimal Kar, Avinash Sonawane, Sushabhan Sadhukhan*
RSC Adv. 12 17821 (2022)

Pharmacological and genetic perturbation establish SIRT5 as a promising target in breast cancer
Yashira L. Negrón Abril, Irma R. Fernandez, Jun Young Hong, Ying-Ling Chiang, Dennis A. Kutateladze, Qingjie Zhao, Min Yang, Jing Hu, Sushabhan Sadhukhan, Bo Li, Bin He, Brenna Remick, Jessica Jingyi Bai, James Mullmann,.... Hening Lin, Robert S. Weiss.
Oncogene 40 1644–1658 (2021)

An in-silico study on selected organosulfur compounds as potential drugs for SARS-CoV-2 infection via binding multiple drug targets
Liya Thurakkal, Satyam Singh, Rajarshi Roy, Parimal Kar, Sushabhan Sadhukhan*, Mintu Porel*
Chem. Phys. Lett. 763 138193 (2021)

Plant-derived natural polyphenols as potential antiviral drugs against SARS-CoV-2 via RNA‐dependent RNA polymerase (RdRp) inhibition: An in-silico analysis
Satyam Singh, Md Fulbabu Sk, Avinash Sonawane, Parimal Kar, Sushabhan Sadhukhan*
J. Biomol. Struct. Dyn. 39 (16) 6249-6264 (2021)

Direct Comparison of SIRT2 Inhibitors: Potency, Specificity, Activity-dependent Inhibition, and On-target Anticancer Activities
Nicole A. Spiegelman, Ian R. Price, Hui Jing, Miao Wang, Min Yang, Ji Cao, Jun Young Hong, Xiaoyu Zhang, Pornpun Aramsangtienchai, Sushabhan Sadhukhan, and Hening Lin.
ChemMedChem. 13 1890-1894 (2018)

Emerging Roles of DHHC-mediated Protein S-palmitoylation in Physiological and Pathophysiological Context
Indranil De, Sushabhan Sadhukhan*
Euro. J. Cell Biol. 97 319-338 (2018)

SIRT7 Is an RNA-activated Protein Lysine Deacylase
Zhen Tong, Miao Wang, Yi Wang, David D. Kim, Jennifer K. Grenier, Ji Cao, Sushabhan Sadhukhan, Quan Hao, and Hening Lin.
ACS Chem. Biol. 12 300-310 (2017)

SIRT7 Is Activated by DNA and Deacetylates Histone H3 in the Chromatin Context
Zhen Tong, Yi Wang, Xiaoyu Zhang, David D. Kim, Sushabhan Sadhukhan, Quan Hao, and Hening Lin.
ACS Chem. Biol. 11 742-747 (2016)

Metabolomics-assisted Proteomics Identifies Succinylation and SIRT5 as Important Regulators of Cardiac Function
Sushabhan Sadhukhan, Xiaojing Liu, Dongryeol Ryu, Ornella Nelson, John Stupinski, Zhi Li, Wei Chen, Sheng Zhang, Robert Weiss, Jason Locasale, Johan Auwerx, and Hening Lin.
Proc. Natl. Acad. Sci. USA. 113 4320-4325 (2016)

High Resolution Metabolomics With Acyl-CoA Profiling Reveals Widespread Remodeling In Response To Diet
Xiaojing Liu, Sushabhan Sadhukhan, Shengyi Sun, Gregory R. Wagner, Matthew D. Hirschey, Ling Qi, Hening Lin, and Jason W. Locasale.
Mol. Cell. Proteomics 14 1489-1500 (2015)

Identification of a Negative Feedback Loop in Biological Oxidant Formation Regulated by 4-Hydroxy-2-(E)-Nonenal
Tonibelle N. Gatbonton-Schwager, Sushabhan Sadhukhan, Guo-Fang Zhang, John J. Letterio, and Gregory P. Tochtrop.
Redox Biol. 2 755-763 (2014)

Glutathionylated 4-Hydroxy-2-(E)-Alkenal Enantiomers in Rat Organs and Its Contributions Towards the Disposal of 4-Hydroxy-2-(E)-Nonenal in Rat Liver
Sushabhan Sadhukhan, Yong Han, Zhicheng Jin, Gregory P. Tochtrop, and Guo-Fang Zhang.
Free Rad. Biol. Med. 70 78-85 (2014)

Modular Isotopomer Synthesis of γ-Hydroxybutyric Acid for a Quantitative Analysis of Metabolic Fates
Sushabhan Sadhukhan, Guo-Fang Zhang, and Gregory P. Tochtrop.
ACS Chem. Biol. 9 1706-1711 (2014)

Catabolism of 4-Hydroxy-2-(E)-Nonenal via Omega- and Omega-1 Oxidation: Stimulated by Ketogenic Diet
Zhicheng Jin, Jessica M. Berthiaume, Qingling Li, Fabrice Henry, Zhong Huang, Sushabhan Sadhukhan, Peng Gao, Gregory P. Tochtrop, Michelle A. Puchowicz and Guo-Fang Zhang.
J. Biol. Chem. 289 32327-32338 (2014)

4-Hydroxy-2-(E)-nonenal (HNE) Catabolism and Formation of HNE Adducts Are Modulated by β-oxidation of Fatty Acids in the Isolated Rat Heart
Qingling Li, Sushabhan Sadhukhan, Jessica M. Berthiaume, Rafael A. Ibarra, Hui Tang, Shuang Deng, Eric Hamilton, Laura E. Nagy, Gregory P. Tochtrop, and Guo-Fang Zhang.
Free Rad. Biol. Med. 58 35-44 (2013)

Metabolomics Coupled to Mass Isotopomer Analysis as a Strategy for Pathway Discovery: Pyrrolyl and Cyclopentenyl Derivatives of the Pro-Drug of Abuse, Levulinate
Stephanie R. Harris, Guo-Fang Zhang, Sushabhan Sadhukhan, Hua Wang, Chuan Shi, Michelle A. Puchowicz, Vernon E. Anderson, Robert G. Salomon, Gregory P. Tochtrop, and Henri Brunengraber.
Chem. Res. Toxicol. 26 213-220 (2013)

Metabolic Characterization of a Sirt5 Deficient Mouse Model
Jiujiu Yu, Sushabhan Sadhukhan, Lilia G. Noriega, Norman Moullan, Bin He, Robert S. Weiss, Hening Lin, Kristina Schoonjans, and Johan Auwerx.
Sci. Rep. 3 2806 (2013)

Metabolism of gamma-Hydroxybutyrate (GHB) in Perfused Rat Livers and Hearts
Guo-Fang Zhang, Sushabhan Sadhukhan, Gregory P Tochtrop, and Henri Brunengraber.
Biochem. J. 444 333-341 (2012)

Metabolomics, Pathway Regulation and Pathway Discovery
Guo-Fang Zhang, Sushabhan Sadhukhan, Gregory P. Tochtrop, and Henri Brunengraber.
J. Biol. Chem. 286 23631-23635 (2011)

Isotopomer Enrichment Assay for Very Short Chain Fatty Acids and Its Metabolic Applications
Kristyen Tomcik, Rafael A. Ibarra, Sushabhan Sadhukhan, Yong Han, Gregory P. Tochtrop, and Guo-Fang Zhang.
Anal. Biochem. 410 110-117 (2011)

Metabolism of Levulinate in Perfused Rat Livers and Live Rats: Conversion to the Drug of Abuse 4-Hydroxypentanoate
Stephanie R. Harris, Guo-Fang Zhang, Sushabhan Sadhukhan, Anne M. Murphy, Kristyen A. Tomcik, Edwin J. Vazquez, Vernon E. Anderson, Gregory P. Tochtrop, and Henri Brunengraber.
J. Biol. Chem. 286 5895-5904 (2011)

Synthesis and Biological Evaluation of Analogues of a Novel Inhibitor of beta-Amyloid Secretion
Enakshi Chakrabarti, Subrata Ghosh, Sushabhan Sadhukhan, Lawrence Sayre, Gregory P. Tochtrop, and Jonathan D. Smith.
J. Med. Chem. 53 5302-5319 (2010)

Using Selective Isotopic Labeling to Quantitate Parallel Metabolic Pathways
Sushabhan Sadhukhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P Tochtrop.
J. Am. Chem. Soc. 132 6309-6311 (2010)

Catabolism of 4-Hydroxyacids and 4-Hydroxynonenal via 4-Hydroxy-4-Phosphoacyl-CoAs
Guo-Fang Zhang, Rajan S. Kombu, Takhar Kasumov, Yong Han, Sushabhan Sadhukhan, Jianye Zhang, Lawrence M. Sayre, Dale Ray, K. Michael Gibson, Vernon A. Anderson, Gregory P. Tochtrop and Henri Brunengraber.
J. Biol. Chem. 284 33521-33534 (2009)

The Syntheses and Applications of beta-Benzylmercaptoethylamine Derivatives
Gregory P. Tochtrop, Sushabhan Sadhukhan, Rik Rani Koner, and Subrata Ghosh.
Tetrahedron 65 10515-10534 (2009)

Lipase Catalyzed Synthesis of Benzyl Acetate in Solvent-Free Medium Using Vinyl Acetate As Acyl Donor
Abir B. Majumder, Bhupender Singh, Debjit Dutta, Sushabhan Sadhukhan, and Munishwar N. Gupta.
Bioorg. Med. Chem. Lett. 16 4041-4044 (2006)